Cytokine secretion and proliferation after allogeneic stimulation of highly purified peripheral T-cell subsets from healthy adults. (A) Cytokine profile of purified DN (▪) and CD4⁺ (□) T cells stimulated with allogeneic mature DCs for 48 hours. A representative result (± SD) of 3 independent experiments is shown. (B) Sorted DN (▪), CD4⁺ (□), and CD8⁺ T cells (▦) were stimulated with irradiated allogeneic PBMCs in the presence or absence of IL-2 (100 U/mL) or IL-2 alone. [³H]TdR incorporation was measured after 4 days of culture. Bars represent the means (± SD) of triplicate cultures. Data represents one of 3 experiments with similar results using cells from 3 different donors.