Figure 3.
IL-4 signaling in GCB-like and ABC-like DLBCL cell lines. GCB-like (SUDHL6, SUDHL4, OCILY19) and ABC-like (OCILY10, OCILY3) DLBCL cell lines were assessed for activation of STAT6 and AKT following IL-4 (100 U/mL) stimulation by immunoblotting cellular lysate protein with anti-pSTAT6 (A) and antiphosphorylated AKT (serine 473; B) antibodies. Unstimulated and IL-4-stimulated Daudi cells are used as a negative and positive control for pSTAT6. MCF7 cells in serum-free medium (24 hours) and in complete medium are used as a negative and positive control for pAKT.