Effects of STAT6 nuclear export inhibitor leptomycin B on nuclear STAT6 and pSTAT6 in the GCB-like and ABC-like DLBCL cell lines. (A) GCB-like (SUDHL6 and SUDHL4) and ABC-like (OCILY10) DLBCL cell lines were stimulated with IL-4 (100 U/mL) for the indicated times with and without 3 hours preincubation with leptomycin B (10 ng/mL). Nuclear protein extract was immunoblotted with anti-pSTAT6, anti-STAT6, and antinucleolin antibodies. Exposure time of every blot is indicated (5 seconds or 1 minute). (B) pSTAT6 densitometry measurements of the demonstrated blots. The results shown represent a fold change of pSTAT6/nucleolin densitometry ratio at each condition compared with the IL-4 stimulation alone at 30 minutes in the same cell line. The solid line indicates IL-4 alone; the dotted line, IL-4 and leptomycin B.