Changes in thymocyte populations in cocultures of CD34⁺ simian BM cells from healthy and uninfected macaques and human thymic stromal cells. (A) Flow cytometry diagrams of a representative coculture for CD34 or CD4 and CD8 expression. On day 0 of coculture, 2 × 10⁴ purified BM CD34⁺ cells were seeded on a stromal monolayer. At each time point, all of the cells present in the culture well were used for flow cytometry. (Bi) Changes in CD34⁺ (•), CD34⁺CD7⁺ (□), or CD3⁺CD4⁺CD8⁺ (○) populations in 4 independent experiments using BM CD34⁺ cells obtained from 4 different cynomolgus macaques. (ii) Changes in CD3⁺ (○) or CD3⁺CD4⁺CD8^- (•) in the same 4 cultures; simian BM CD34⁺ cells were seeded on day 0.