Inhibitory effect of IMD-0354 on cell proliferation of HMC-1 cells. (A) Molecular formula of IMD-0354. (B) Estimated docking structures of ATP or IMD-0354 with IKKβ. (C) Inhibitory effect of IMD-0354 on NF-κB activity was examined by a reporter assay with the use of HMC-1 cells transfected with pNF-κB-TA-Luc plasmid. Luciferase activity was reduced in a dose-dependent manner by treatment with IMD-0354 for 24 hours. Each value represents the mean ± SE at each time point of 3 different experiments with duplication. ^*P < .01 compared to medium alone. (D) EMSA revealed that more than 1 μM IMD-0354 inhibited the translocation of NF-κB to the nucleus after treatment for 24 hours. Results shown are representative of those of 5 different experiments.