Suppressive effect of IMD-0354 on cell cycle progression of HMC-1 cells. (A) HMC-1 cells were incubated with IMD-0354 or STI571 for 24 hours, and the cell cycle was analyzed by PI staining. The percentage of cells with hypodiploid DNA contents was measured. Data represent the mean ± SE of 3 different experiments with duplication. (B) HMC-1 cells were treated with IMD-0354 or STI571 for 24 hours, and DNA was collected as described in “Materials and methods.” A ladder is marked in cells treated with 1 μM IMD-0354 for 24 hours. Results shown are representative of those of 3 different experiments. (C) HMC-1 cells were treated with indicated doses of IMD-0354 for 24 hours. Early apoptotic cells were detected by annexin V-FITC binding and flow cytometric analysis. Dots in the right under compartment were only annexin V-FITC-positive cells. Data represent the mean ± SE of 4 different experiments. ^*P < .01 compared with medium alone.