Chondroitinase treatment inhibits adhesion, migration, and invasion of HUVECs to the 5 EGF (E/1-5-RGD-FL) but not the 3 EGF (E/1,2,5-RGD-FL) splice variant of CD97. Assays were designed as outlined in previous figures. Cells were left untreated or exposed to 150 mU/mL chondroitinase ABC (Sigma-Aldrich, Saint Louis, MO) for 30 minutes at 37°C before being loaded into the assay system. Results shown are representative of at least 5 replicates. Data show mean number of cells per ×200 microscopic field plus or minus SEM.