Analysis of the subcellular localization of the various BCR components by immunofluorescence microscopy. Fixed and permeabilized CLL B cells were incubated with various combinations of mAbs as follows: anti-μ/anticalnexin (A), anti-CD79a/anticalnexin (B), and anti-CD79b/anti-TGN46 (C). Red and green images were collected and merged, with yellow coloration indicating colocalization. These images were obtained with B cells from patient 2 (Table 1).