Figure 1.
Localization of HCs in colon26 tumor tissues. (A-D) Sections of colon26 tumor tissues from tumors with a volume of (A) 30 to 40 mm3, (B) 60 to 70 mm3, (C) 100 and 120 mm3, and (D) more than 500 mm3 were stained with anti–PECAM-1 mAb (dark blue) and anti-CD45 mAb (red). The dotted line (A-C) shows the border between the fibrous cap surrounding the tumor mass and the tumor mass. The area beneath the dotted line is the tumor mass. (E-H) Sections from colon26 tumors of 60 to 70 mm3 that were stained with anti-CD11b (Mac-1) (E), anti-B220 (F), a mixture of anti-CD4 and anti-CD8 (G), or anti–Ly-76 (Ter119) (H) mAb. Insets in panels A-D show high-power views of the area indicated by box. Bar indicates 50 μm.
