Effect of anti-CD49d mAb on neutrophil mobilization stimulated by MIP-2. Using the in situ perfusion system of the rat femoral bone marrow, anti-CD49d mAb, Max68P, (10 μg/mL) or an isotype-matched control mAb (10 μg/mL) was infused for a total of 30 minutes, and MIP-2 (1 nM) or vehicle (PBS/0/1% BSA) was infused for 10 minutes, as indicated by the solid bars. (Left) The kinetics of neutrophil mobilization (•, MIP-2 + vehicle; ▴, MIP-2 + anti-CD49d mAb; ○, vehicle + IgG; ▵, vehicle + CD49d mAb) and (right) the total number of neutrophils mobilized are shown on the right-hand axis, mean ± SEM (n = 5 separate perfusions). ^*P < .05.