CHIR-258 inhibits viability of KMS11 cells in the presence of IL-6, IGF-1, and BMSCs. (A) KMS11 cells were cultured in with DMSO (□) and with 100 nM (▦) or with 500 nM (▧) CHIR-258 in the presence of 30 ng/mL FGF, 50 ng/mL IL-6 or 50 ng/mL IGF-1. Cell viability after 48 hours was assessed by MTT assay. (B) BMSCs, KMS11 cells, or BMSCs together with KMS11 were cultured with DMSO (□), 100 nM (▦), or 500 nM (▧) CHIR-258 and viability after 96 hours was assessed by MTT assay. The data represent means of quadruplicate cultures ± SD.