Plag1 and PLAGL2 cooperate with Cbfb-MYH11 to induce AML in mice. (A) Kaplan-Meier survival curve of mice that received transplants with bone marrow cells expressing: Cbfb-MYH11 and MIG-Plag1 (thick dashed line), Cbfb-MYH11 and MIG-PLAGL2 (thick solid line), Cbfb-MYH11 and MIG (thin solid line), MIG-Plag1 (thin dashed line), or MIG-PLAGL2 (dotted-dashed line). (B) Representation of the MIG-Plag1/L2 provirus. The LTRs (▸), coding sequence for Plag1/L2 (sequence encoding Plag1 or PLAGL2) and GFP (open arrows), internal ribosome entry site (IRES; ▥), probe (thick line), HindIII restriction sites (H), genomic (thin line) and viral (double thin line) sequences, are detailed. (C) Representative Southern blot analysis of transplantation of leukemic cells. DNA from PLAG-associated primary leukemia (lanes 1, 6, 9, 11), secondary transplanted leukemias (lanes 2, 3, 7, 8, 10, 12, 13), and tertiary transplanted leukemias (lanes 4 and 5) were probed with the retroviral GFP sequence. Thin arrows indicate which primary leukemic cells were used for secondary and tertiary transplants. Asterisk shows a band that represents the clonal expansion of a population of leukemic cells that became predominant in mice that receive transplants. The expected molecular weight of expected fragments (in kb) is indicated at the left. (D) Pathologic characteristics of leukemic cells. Wright-Giemsa staining of (× 10 magnification) normal (panel i) and leukemic (panel iii) bone marrow, normal (panel ii) and leukemic spleen (panel iv), and representative blastlike (panel v) and monocytic-like (panel vi) cells (× 100 magnification). Note area with lymphoid (A), red (B), megakaryocytes (C), and leukemic (D) cells.