Detection of bait-prey interactions in TF-1 cells. EpoR bait constructs, as indicated, in combination with the PLCγ(2 × SH2) prey and the pXP2d2-rPAP1-luci reporter were electroporated in TF-1 cells. After removal of GM-CSF for 24 hours, cells were stimulated with Epo or were left untreated (NS, not stimulated). Average values for relative luciferase activities (x-fold increase, luciferase values obtained from stimulated cells with respect to values derived from untreated cells) are shown. A representative experiment from 3 is shown.