Cytokine profiling of LDSAC- and HDSAC-conditioned growth medium. Mouse cytokine antibody arrays were performed using TransSignal RayBio Mouse Cytokine Antibody Array Kit (MA6050) according to the manufacturer's instructions. Membranes were incubated with LDSAC- or HDSAC-derived medium for 24 hours. Blots were developed and subsequently treated according to “Materials and methods.” Autoradiography was then performed, and intensities were measured by densitometric analysis. Following normalization of the data, with positive controls set at 100% and negative controls at 0, data were plotted and significance considered among the tested cytokines to reflect P < .05. GCSF indicates granulocyte colony stimulating factor; GM-CSF, granulocyte macrophage colony stimulating factor; INF, interferon; and IL, interleukin. Error bars indicate ± standard deviation.