Developmental independence of pDCs on RAG1, RAG2, IL-7Rα, or Pax5. (A) pDC incidence in marrow of RAG1, RAG2, CD127/IL-7Ra, and Pax5 knock-out mice. Results are presented as the mean ± SD of 3 different experiments. (B) pDCs were sorted from BM of 12-day-old Pax5^-/- and littermate control mice. RT-PCR analysis for expression of the indicated genes was performed with 5-fold serial dilutions of cDNA prepared from these cells. The cDNA input was normalized according to the expression of the control HPRT gene.