NE, MMP-9, and active MMP-9 levels in the MNC-conditioned media. (A) Levels of NE in media conditioned by MNCs isolated from the PB of healthy subjects (n = 13), patients with PV (n = 14), or patients with IM (n = 17) were assayed by ELISA. Each point represents the mean concentration of NE in media conditioned by the MNCs from individual patients with IM and PV as well as healthy control subjects assayed in duplicate. The bar represents the mean of each sample group. The levels of NE in the MNC-conditioned media from both patients with PV (P < .05) and patients with IM (P < .001) were significantly greater than that of healthy control subjects. There was no significant difference between the levels of NE in media conditioned by MNCs from patients with IM and PV (P > .05). (B) Levels of MMP-9 in media conditioned by MNCs isolated from normal PB (n = 15), PV PB (n = 14), and IM PB (n = 21). Each point represents the mean concentration of MMP-9 in media conditioned by the MNCs from individual patients with IM and PV as well as healthy control subjects assayed in duplicate. The bar represents the mean of each sample group. The levels of MMP-9 in the MNC-conditioned media from healthy control subjects were not significantly different than that in conditioned media prepared with MNCs from both patients with PV and patients with IM (P > .05). (C) Levels of endogenous active MMP-9 in media conditioned by MNCs isolated from normal PB (n = 10), PV PB (n = 9), and IM PB (n = 12). Each point represents the mean concentration of endogenous active MMP-9 in media conditioned by the MNCs from individual patients with IM and PV as well as healthy control subjects assayed in duplicate. The bar represents the mean of each sample group. The levels of endogenous active MMP-9 in the MNC-conditioned media from healthy control subjects were not significantly different from that in conditioned media prepared with MNCs from both patients with PV and patients with IM (P > .05).