CsA and tacrolimus do not inhibit phagocytic activity of DCs. DC2.4 cells were incubated with microspheres containing both OVA and FITC in the presence or absence of CsA (3000 nM) or tacrolimus (100 nM) for 2 hours, washed, harvested, and then analyzed in a flow cytometry. Shaded histograms represent the phagocytic activity of DCs in the absence of CsA or tacrolimus, and thin line histograms represent the phagocytic activity of DCs in the presence of CsA or tacrolimus. DCs that were not incubated with FITC-labeled microspheres were shown as a dotted histogram.