Effect of blocking NF-κB activation on in vivo TNF-α–induced TM repression. The lungs of mice were transduced with no virus or 109 pfu of either AdNull or AdIκBsr by intratracheal instillation. Four days later they were challenged with either PBS (A-B, ▪) or 20 μg human TNF-α (A-B, ▦) by intratracheal instillation. (A) Pulmonary NF-κB activity measured in whole lung nuclear extracts by ELISA. *P < .007 for AdIκBsr versus no virus or AdNull controls challenged with TNF-α. (B) Pulmonary TM gene expression in whole lung extracts determined by real-time PCR. #P < .04 for AdIκBsr versus no virus, and AdNull controls were challenged with TNF-α. All values are the mean ± SEM of 3 experiments. (C) Photomicrographs of TM immunostaining 16 hours after exposure to either PBS or TNF-α.
