Effect of p300 overexpression on TNF-α–induced TM repression. HUVECs were transduced with either AdNull, Adp300, or AdIκBsr (MOI = 100 pfu/cell) prior to stimulation without (▪) or with 100 ng/mL human TNF-α (▦). (A) TM gene expression, normalized to CD31, was determined by real-time PCR 16 hours after TNF-α stimulation. (B) Activated protein C formation was determined by in situ chromogenic assay 24 hours after TNF-α stimulation. Values are the mean ± SEM of 3 experiments. #P < .004 for Adp300 versus AdNull and control groups stimulated with TNF-α. ^*P < .01 for AdIκBsr versus AdNull and control groups stimulated with TNF-α.