CC-4047 enhances secretion of myeloid-specific cytokines. CD34⁺ cells were cultured with SCF plus IL-3 plus IL-6 as indicated in Figure 2A in the presence of thalidomide (100 μM; ), CC-4047 (100 μM; ▪), or 0.1% DMSO (control; □). Supernatants were analyzed for cytokine secretion (picograms per milliliter) after 1, 3, and 6 days of treatment by Bio-Plex Cytokine Assay. Intra-assay variability, expressed as coefficient of variation (CV = standard deviation divided by the mean), was calculated based on determining quadruplicates of standards. The CV for each experiment was less than 10%.