Figure 1.
Phosphotyrosine-containing proteins in c-Cbl immunoprecipitates of platelets stimulated with convulxin. Platelets were stimulated with 100 ng/mL convulxin for the indicated times and lysed with NP-40 lysis buffer. Proteins were immunoprecipitated with an antibody to c-Cbl and probed with an antibody to phosphotyrosine. Blots were analyzed on a Fuji LAS-1000 plus imaging system. This experiment and those shown in all other figures are representative of at least 3 similar experiments. Stds indicates protein molecular weight standards.