Figure 3. Podocalyxin becomes... | American Society of Hematology

Figure 3.

$Figure 3. Podocalyxin becomes progressively more restricted to hematopoietic progenitors during embryonic development. E12 YS (A), E15 FTL (B), and 1-day BM (C) were stained with anti-PCLP1 plus antirat IgG–Alexa-fluor 488, followed by Ter119-biotin plus streptavidin-APC and CD71-PE, CD45-PE, or Mac-1–PE. PCLP1low and PCLP1high cells were gated as indicated, and coexpression of hematopoietic markers in each population was assessed by 3-color FACS analyses. Solid curves indicate specific staining with the indicated antibodies. Open curves indicate isotype-matched control stains. The “gated” percentages of cells in each quadrant are indicated. Red boxes indicate colony-forming cells from E12 YS. Blue boxes indicate the hematopoietic precursor fraction of 15-day FTL.$

Podocalyxin becomes progressively more restricted to hematopoietic progenitors during embryonic development. E12 YS (A), E15 FTL (B), and 1-day BM (C) were stained with anti-PCLP1 plus antirat IgG–Alexa-fluor 488, followed by Ter119-biotin plus streptavidin-APC and CD71-PE, CD45-PE, or Mac-1–PE. PCLP1^low and PCLP1^high cells were gated as indicated, and coexpression of hematopoietic markers in each population was assessed by 3-color FACS analyses. Solid curves indicate specific staining with the indicated antibodies. Open curves indicate isotype-matched control stains. The “gated” percentages of cells in each quadrant are indicated. Red boxes indicate colony-forming cells from E12 YS. Blue boxes indicate the hematopoietic precursor fraction of 15-day FTL.

This Feature Is Available To Subscribers Only