Figure 3.
Quantitative RT-PCR for RE-IIBP on BMMCs. The relative expression level of RE-IIBP from a panel of unfractionated BMMCs from t(4;14)POS and t(4;14)NEG patients was determined by the ΔΔCt analysis method using the Raji cell line as a reference expression level of 1. Patients positive for the translocation are denoted by a black line along the x-axis and they are grouped into their respective breakpoint types as indicated. ▦ represents t(4;14)POS FGFR3 nonexpressing patients. Patients, t(4;14)POS, were selected on the basis of a BM plasmacytosis more than 35%, and then best match t(4;14)NEG samples were selected based on sex, M-protein isotype, bone marrow plasmacytosis, and age. Error bars represent the standard deviation.