Pim-2 and Akt cooperate to promote increases in cell size, cell survival, and tumorigenesis in transgenic animals. (A) Lysates prepared from thymuses of littermate control, Akt1 transgenic (Akt TG), Pim-2 transgenic (Pim-2 TG), and double-transgenic animals were probed for the expression of phosphothreonine 37/46 4EBP-1 (p-37/46 4EBP-1), phospho-threonine 70 4EBP-1, 4EBP-1, phospho-serine 209 eIF4E (p-209 eIF4E), eIF4E, Pim-2, phospho-serine 473 Akt (p-473 Akt), and Actin. A nonspecific band is observed above Pim-2 in all lanes of the sixth blot. (B) Cell size measurements are shown from peripheral T cells isolated from wild-type (WT), Pim-2 transgenic (Pim-2), Akt transgenic (Akt), and compound transgenic (Akt + Pim-2) cells. Data represent mean ± SD of 3 independent samples. ^*Significance (P < .05) compared with wild-type cells (analysis of variance [ANOVA]). (C) Viability is shown for transgenic T cells 24 hours after isolation. Mean ± SD of triplicate measurements are depicted. ^*Significance (P < .05) compared with wild-type cells (ANOVA). (D) Incidence of lethal lymphoma in transgenic mice of the following genotypes: WT, Pim-2 TG, Akt TG, and Akt TG + Pim-2 TG. Ten mice are included for each genotype.