Figure 4.
IgG-secreting cells formed in cultures supplemented with IFN-γ showed enhanced migration toward CXCL9. Plasma-cell differentiation was induced on sorted CXCR3+ or CXCR3- peripheral blood B cells in the presence or absence of IFN-γ. After 8 days of culture, migration of IgG+ antibody-secreting cells (ASC) toward 100 nM CXCL9 was analyzed by Transwell chemotaxis assays. IgG-secreting cells were quantified by ELISPOT. Each dot represents the percentage of migrating IgG-secreting cells of one experiment. Differences between frequencies of migrating cells from cultures with and without IFN-γ are statistically significant (P < .05). Horizontal bars indicate the median value.