Effect of CYC202 on protein expression in B-CLL. (A) Western blotting showing effects of incubation with 5 μg/mL CYC202 on the expression of p21^WAF and p53 proteins in representative ATM/TP53 wild-type, ATM mutant, and TP53 mutant B-CLL cells. p53-actin ratio was determined by densitometry and is given above the Western blot. (B) Effect of the same treatment on the cleavage of PARP1, procaspase-3, and procaspase-7. Arrows indicate cleaved products of PARP1 and caspase-3. ATM/TP53 wild-type cells were treated with CYC202 for 0, 1, 2, 3, 4, 5, 6, and 24 hours; ATM mutant cells, for 0, 2, 10, and 18 hours; and TP53 mutant cells, for 0, 2, 4, 6, 8, 10, and 24 hours. Actin was used as a loading control.