Figure 3.
Figure 3. Analyses of Tie2 protein levels in embryos and cultured endothelial cells by Western blotting. (A) Analyses of Tie2 protein levels in wild-type (wt) and Pik3ca-/- (-/-) tissues. Tie2 is significantly reduced in Pik3ca-/- embryos and yolk sacs. Akt expression is unaltered, but its phosphorylation level (P-Akt) is reduced in Pik3ca-/- tissues. Western blots are representative of 3 repeats. (B) Effect of p110α knock-down on Tie2 expression. Lane 1, LacZ siRNA as control; lane 2, 80 pmol/mL of p110α SmartPool siRNA; lane 3, 20 pmol/mL of p110α SmartPool siRNA. (C) Bar graph representation of results shown in panel B. p110α and Tie2 protein levels are shown as percentages of remaining protein levels relative to lacZ siRNA control. Numbers on the horizontal axis correspond to lane numbers in panel B.

Analyses of Tie2 protein levels in embryos and cultured endothelial cells by Western blotting. (A) Analyses of Tie2 protein levels in wild-type (wt) and Pik3ca-/- (-/-) tissues. Tie2 is significantly reduced in Pik3ca-/- embryos and yolk sacs. Akt expression is unaltered, but its phosphorylation level (P-Akt) is reduced in Pik3ca-/- tissues. Western blots are representative of 3 repeats. (B) Effect of p110α knock-down on Tie2 expression. Lane 1, LacZ siRNA as control; lane 2, 80 pmol/mL of p110α SmartPool siRNA; lane 3, 20 pmol/mL of p110α SmartPool siRNA. (C) Bar graph representation of results shown in panel B. p110α and Tie2 protein levels are shown as percentages of remaining protein levels relative to lacZ siRNA control. Numbers on the horizontal axis correspond to lane numbers in panel B.

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