Array CGH profile of MCL. (A, left) Array CGH profile of MCL and other B-NHL subtypes. Patients with MCL are ordered from left to right according to the length of OS time, ranging from 0 to 115 months. FCL indicates follicle center lymphoma; BL, Burkitt lymphoma; DLBCL, diffuse large B-cell lymphoma; MZL, marginal zone lymphoma. (Right) Genomic delineation of the deletions of 1p21.2-p21.3 and of 10p14 with amplification of 10p12 (BMI1 gene locus). Genomic gains are shown in red, genomic losses in green, and regions with normal DNA copy number in black. (B) Multicolor FICTION analysis of case no. MCL114. BMI1 amplification was more common in apparently proliferating cyclin D1–positive MCL cells (38 of 50 cells; 76%) as adjudged by Ki-67 staining, whereas those cyclin D1–positive cells negative for Ki-67 showed this gene amplification less commonly (14 of 50 cells; 28%). A Zeiss Axioskop 2 fluorescence microscope (Zeiss, Gottingen, Germany) equipped with appropriate filter sets was used.