OSU-03012-mediated Akt dephosphorylation does not sensitize 32D and untransfected Ba/F3 cells to imatinib-induced apoptotic death. (A) Dose-response curves obtained by MTS assays after 48-hour exposure to a combination of 5 μM OSU-03012 and varying concentrations of imatinib mesylate. Each data point represents mean ± SD. (B) Effect of 5 μM OSU-03012 on Thr³⁰⁸ phosphorylation of Akt in 32D (•) and Ba/F3 (▴) cells after 36-hour exposure. The immunoblots are representative of 3 independent experiments.