Subcellular localization and organization of PfEMP1-GFP. The 3D7-K60TmATSs-GFP (A-B), 3D7-K60Tm(ama1)ATSs-GFP (C), and 3D7-K119TmATS-GFP (D-F) transfectants were labeled with αGFP antibody. In each case the chimeric protein was observed within the parasite, in aggregates at and near Maurer clefts (arrowheads), and near the surface of the RBC. Gold particles were also often observed associated with electron dense aggregates in the red blood cell cytosol (D-E, arrowheads). Bars represent 100 nm.