Figure 2.
Figure 2. Far-UV CD spectra of recombinant wild-type and L131P mutant P5′N-1. (A) At 25°C, wild type (solid line) and L131P (dashed line) showed similar spectra with a maximum at 192 nm (18 292 ± 302 deg cm2 dmol-1 and 18 092 ± 212 deg cm2 dmol-1, respectively) and double minima at 208 nm (-9854 ± 128 deg cm2 dmol-1 and -9671 ± 113 deg cm2 dmol-1) and 220 nm (-9793 ± 105 deg cm2 dmol-1 and -9871 ± 101 deg cm2 dmol-1). (B) At 37°C, the wild type (solid line) displayed a spectrum identical to that recorded at 25°C, whereas L131P (dashed line) lost the double minima at 208 and 220 nm and had a reduced maximum at 192 nm (14 252 ± 282 deg cm2 dmol-1).

Far-UV CD spectra of recombinant wild-type and L131P mutant P5′N-1. (A) At 25°C, wild type (solid line) and L131P (dashed line) showed similar spectra with a maximum at 192 nm (18 292 ± 302 deg cm2 dmol-1 and 18 092 ± 212 deg cm2 dmol-1, respectively) and double minima at 208 nm (-9854 ± 128 deg cm2 dmol-1 and -9671 ± 113 deg cm2 dmol-1) and 220 nm (-9793 ± 105 deg cm2 dmol-1 and -9871 ± 101 deg cm2 dmol-1). (B) At 37°C, the wild type (solid line) displayed a spectrum identical to that recorded at 25°C, whereas L131P (dashed line) lost the double minima at 208 and 220 nm and had a reduced maximum at 192 nm (14 252 ± 282 deg cm2 dmol-1).

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