Proliferation kinetics of single G₀CD34⁺CD38^–/lo cells recorded on days 5 and 7. Single G₀CD34⁺CD38^–/lo cells were delivered into individual round-bottomed 96-well plates prepared with CM and 1 of the 7 cytokine cocktails depicted on the x-axis (Table 1 presents the details). Cell number in each well was counted on days 3 and 5 and the percentage of wells containing more than 2 cells on day 3 (red bars) or just one cell on day 5 (blue bars) were plotted in panel A. (B-C) Similarly, cell number in each well was counted on days 3, 5, and 7 and the percentage of wells (z-axis) containing clones composed of 1 to 8 cells (yellow bars), 9 to 32 cells (red bars), and more than 32 cells (green bars; y-axis) was calculated for each group on days 5 (B) and 7 (C). Between 288 and 984 clones were analyzed for each cytokine combination.