Figure 6.
![Figure 6. The M1.2 peptide deduced from the signal sequence of the MUC1 protein is presented TAP independently. To analyze if a functional TAP is required for the presentation of HLA class I epitopes, autologous DCs were cotransfected with MUC1 and ICP47 RNA (MUC1 alone, ×; ICP47 alone, ♦; both, ▴) and used as targets for the recognition by MUC1-specific CTLs (M1.1 [A] and M1.2 [B]). DCs pulsed with the synthetic cognate MUC1 peptide (▪) or electroporated with irrelevant EGFP RNA (×) were included as controls.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/8/10.1182_blood-2004-09-3556/6/zh80080576850006.jpeg?Expires=1735506053&Signature=YcP6jZQ-qY9Xib5tSBfQoZrruYqoeEaYewQ8dM8v4P3oAldZ1kJ5RXCTwUZCyAKT9XI3uWQ4flWXMAm3CNm7UF~d2U1PJBehlX9b~Xv3Ciz5RhA~0JLb4sp2-CYJTV~EkK3TMf2ywXENDMSRDHMoGNrjYjVX~fjTh2Ge4u-rUybIe6fr9HxWzeNHEAuHuIJWy42q1RthzIkc2JbEZZR-KdSDdeArwzbfkJXii0xyNNe3G3glPT7ApQU5PwRVYRtBoE2tpftb~CVEBe5AapAoK2q70YD2L3i74bpJKJfNJi-vzPert8VUgPHi~VqVclZiBOXEPkn-A3~pBHbUb-LchQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The M1.2 peptide deduced from the signal sequence of the MUC1 protein is presented TAP independently. To analyze if a functional TAP is required for the presentation of HLA class I epitopes, autologous DCs were cotransfected with MUC1 and ICP47 RNA (MUC1 alone, ×; ICP47 alone, ♦; both, ▴) and used as targets for the recognition by MUC1-specific CTLs (M1.1 [A] and M1.2 [B]). DCs pulsed with the synthetic cognate MUC1 peptide (▪) or electroporated with irrelevant EGFP RNA (×) were included as controls.
