Figure 5.
IL-6 induced translocation of CD45RB but not CD45RA to lipid rafts in IL-6Rα-transformed B cells. (A) KUS cells transduced with IL-6Rα (KUS IL-6Rα) or with vector alone (KUS mock) were stained with anti-CD126 PE and analyzed by flow cytometry. The percentage of positive cells relative to the control is given in each histogram. (B) KUS mock and KUS IL-6Rα cells with or without IL-6 treatment were subjected to sucrose density fractionation, followed by immunoblotting with the antibodies indicated.