Lymphocytes from Vav1-deficient mice do not show defects on SDF-1α–induced cell migration and F-actin increase. (A) Mouse lymphocytes were isolated from spleen or lymph nodes (LN) of wild-type (WT; □) or Vav1^–/–-deficient mice (KO; ▪) and allowed to migrate for 3 hours in 3-μm pore diameter Boyden-modified migration chambers in the presence of 10 nM SDF-1α. Transmigrated as well as control cells were stained for CD3 or B220 surface molecules respectively and quantified by flow cytometry. Results correspond to the mean ± SEM of the percentage of transmigrated cells per condition. Wild type, n = 8; Vav1^–/–-deficient, n = 8. Experiments were performed in triplicate. (B) Mouse lymphocytes were isolated from spleen or lymph nodes (LN) of wild type or Vav1^–/–-deficient mice, stimulated with 10 nM SDF-1α for 20 seconds (▪) or not (□), stained for F-actin with Alexa488-conjugated phalloidin, and F-actin content evaluated by flow cytometry. Results correspond to the mean fold induction ± SEM. Wild type, n = 8; Vav1^–/–-deficient, n = 8. Experiments were performed in triplicate.