Oncogenic as well as dominant negative Vav impairs SDF-1α–induced human PBL migration. Human PBLs were nucleofected with the indicated GFP-fusion proteins (□, GFP; ▦, Vav WT; ▦, Vav Δ1-186; and ▪, Vav L213Q) and after 24 hours were allowed to migrate for 3 hours in 3-μm pore diameter Boyden-modified migration chambers in the presence of 10 nM SDF-1α. Cells were gated and analyzed according to their expression of the GFP-fusion protein. Results correspond to the mean ± SEM of the percentage of transmigrated cells per condition in 4 independent experiments performed in triplicate; NO TR, nontransfected.