Analyses of structure and expression of construct HS-40/βpr/γ gene in transgenic mice. Four lines (A-D) carrying this transgene were established. (Top) Structure analysis. DNA from 2 individual animals of each line was digested with NcoI, which cuts once within the construct, and analyzed by Southern blotting with the use of a probe for HS-40. The arrow to the left indicates the 1.7-kb repetitive fragments generated from head-to-tail repeats of the construct. Other bands were from the end fragments containing the HS-40 fragment of the construct and various sequences from the mouse genome. (Bottom) RNA analysis. Total RNA was prepared from the blood of 3- to 4-week-old mice, and RNase protection assay was performed with the use of RNA probes for human γ-globin (Hu γ) and mouse α-globin mRNAs (Mu α). The leftmost lane contains control RNA from cells expressing human γ-globin mRNA at 1.5% of mouse α-globin mRNA.