In vitro migration and IL-12p70 secretion of OX40L mRNA–transfected DCs. (A) Migration of immature DCs (iDC), OX40L mRNA–transfected immature DCs (iDC + OX40L), OX40L mRNA–transfected and cytokine cocktail (without PGE₂)–matured DCs (iDC + CC + OX40L), immature DCs matured with cytokines plus PGE₂ (iDC + CC/PGE₂), or OX40L mRNA–transfected and cytokine cocktail/PGE₂–matured DCs (iDC + CC/PGE₂ + OX40L) toward a MIP-3β gradient in transwell assays with SD (error bars) calculated from triplicate wells. (B) The same cell preparations were analyzed for secretion of bioactive IL-12p70 by ELISA. Results are presented as mean percentage of migrated cells with SD calculated from triplicate wells.