hOSCAR ligation induces expression of maturation markers on mono-DCs. Immature mono-DCs were stimulated by coated control IgG (MOPC21, anti-CD13), anti-hOSCAR whole mAb or F(ab′)2, soluble anti-hOSCAR, or 10 ng/mL LPS. Polymixin B was added to some of the cells cultured in the presence of anti-hOSCAR mAb or LPS. After 24 hours of incubation, cells were analyzed by flow cytometry for CD86 expression (A) or for the indicated markers (B). Numeric values indicate the specific mean fluorescence intensity of the staining (for shaded histograms). The dotted line shows the binding of an isotype control mAb to the cells. Data shown are representative of 3 experiments. (C) Freshly isolated blood CD11c+ DCs were cultured in the presence of coated MOPC21, anti-hOSCAR, or 10 ng/mL LPS. After 24 hours of culture, cells were analyzed by flow cytometry for the indicated markers. The dotted line shows the binding of an isotype control mAb to the cells. Data shown are representative of 6 experiments.
