BCA-1 enhances agonist-induced migration in CCR7-transfected cells. (A,C) Cell migration of CCR7-transfected cells was evaluated in Boyden microchambers. Chemotaxis was induced by SLC (circles) or ELC (diamonds) in the presence (filled symbols) or absence (open symbols) of 1 μM BCA-1. Mean ± SD of 3 independent experiments is shown. (B,D) Chemotaxis induced by 10 nM SLC (circles) or 10 nM ELC (diamonds) in the presence of increasing BCA-1 concentrations. In the absence of SLC or ELC, 1 μM BCA-1 alone does not induce migration of CCR7-transfected cells (▵). Mean ± SD of 3 independent experiments is shown. Cell count was performed in 5 high-power fields (5 hpf). Percentages of maximal migration were calculated taking as 100% the number of cells migrated to 100 nM SLC.