Histology of normal dog muscle 8 weeks after intravascular vector delivery by isolated limb perfusion. (A) Anatomy of dog hindlimb showing sites of biopsy. (B-F) Immunofluorescence staining for canine F.IX at multiple sites as indicated, showing extensive positive staining. (G-H) Hematoxylin and eosin staining of same samples. For all panels, original magnification ×100.