Figure 6.
Figure 6. Induction of DNA damage-dependent G2M delay in FLT3-ITD-TKD–transformed cells. (A) Ba/F3 FLT3-ITD- and ITD-D835Y–expressing cells (untreated and treated for 24 hours with 0.03 μg/mL of daunorubicin) were analyzed by flow cytometry after staining of DNA with propidium iodide. Filled bars indicate G0/1; light gray bars, S; and dark gray bars, G2/M. (B) Ba/F3 FLT3-ITD and ITD-D835Y cells were incubated with daunorubicin in the absence (▪) or in the presence of 5 μM of SU5614 (▦) for 24 hours, and cell cycle analysis after staining with propidium iodide was performed. Values represent means and standard deviations from 3 independent experiments.

Induction of DNA damage-dependent G2M delay in FLT3-ITD-TKD–transformed cells. (A) Ba/F3 FLT3-ITD- and ITD-D835Y–expressing cells (untreated and treated for 24 hours with 0.03 μg/mL of daunorubicin) were analyzed by flow cytometry after staining of DNA with propidium iodide. Filled bars indicate G0/1; light gray bars, S; and dark gray bars, G2/M. (B) Ba/F3 FLT3-ITD and ITD-D835Y cells were incubated with daunorubicin in the absence (▪) or in the presence of 5 μM of SU5614 (▦) for 24 hours, and cell cycle analysis after staining with propidium iodide was performed. Values represent means and standard deviations from 3 independent experiments.

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