Figure 2.
![Figure 2. sPLA2 induces AA release from DC membranes. Day-5 DCs were labeled overnight with [3H]-AA, washed, reseeded in triplicate, and stimulated as indicated for 90 minutes. Supernatants and cell pellets were harvested and radioactivity was determined by scintillation counting. Based on the distribution of radioactivity the percentage of total radioactivity released from cellular membranes into the supernatant was calculated. Results from 1 of 2 almost identical experiments are shown. Values are mean values of quadruplicate measurements ± SD.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/9/10.1182_blood-2004-08-3001/6/zh80090577940002.jpeg?Expires=1735513031&Signature=Gat5Qbq~dVUFJ5kXyYPf9jLgYOp1p9WFxg3dAZYmDsiMgefkAJW8rKCXevxkPeQUuZjm2bZd~~~3ApZ7zbCO70xb4WWQ-SZFlzf~A0Hd3o40LFzWSfHP3OBkRXNjKc~Tq~~nRM0eiYBcYXhb26elPU3En1JKewYqqmB6ETaRAdZpkjax8kbP9TClRc4g7SiaiCXuCy1Tha5LKPLxWdqMeAsCECo8kVJuoauYMvdnyAWBMx-F7QnlIQBUN5dP-9QIQ4wAeOEKu3EK8--L9Om6bcQS-DaKZMxc4K6Of6uqQ5UvvUmxTUZ~x8~XkoLzZkRfzasy7JlXG8We4wHid1i2kg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
sPLA2 induces AA release from DC membranes. Day-5 DCs were labeled overnight with [3H]-AA, washed, reseeded in triplicate, and stimulated as indicated for 90 minutes. Supernatants and cell pellets were harvested and radioactivity was determined by scintillation counting. Based on the distribution of radioactivity the percentage of total radioactivity released from cellular membranes into the supernatant was calculated. Results from 1 of 2 almost identical experiments are shown. Values are mean values of quadruplicate measurements ± SD.
