sPLA2 induces DC maturation. Day-5 DCs were treated for 48 hours in the presence of FCS or LPD-FCS with sPLA2 alone (10 μg/mL) or in combination with TNF-α (100 U/mL) plus IL-1β (1 ng/mL). Cells were stained with monoclonal antibodies specific for CD83 or CD86 and analyzed with a flow cytometer. The percentage of CD83⁺ cells (A) and the MFIs of CD83 (▪) and CD86 (▦) (B) are shown. Results from one experiment representative of 6 independent experiments are shown. LPD indicates lipoprotein-deficient; data are mean values of triplicate measurements ± SD.