Dose response and time course of increased E-selectin RNA expression by PF4. (A) RPA of E-selectin RNA in HUVECs exposed to increasing concentrations of PF4. GAPDH was also measured as an internal control. (B) Relative expression of E-selectin RNA from the experiment in panel A as compared to GAPDH. Data were quantitated as described in “Materials and methods.” (C) RPA demonstrating time course of E-selectin expression after exposure to PF4. (D) Quantitation of data in panel C. Data shown are representative of 3 independent experiments.