Figure 6.
![Figure 6. Specificity of the NF-κB–binding site. EMSAs were performed using nuclear protein from HUVECs and biotin-labeled probes (WT [wild-type] oligo, biotin or Mut oligo, biotin) as described in Figure 5. Symbols above each lane (+/-) denote the presence or absence of various reagents. For competition experiments (lanes 4 and 6), a 50-fold excess of unlabeled oligo (WT oligo or Mut oligo) was used. Data are representative of at least 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/105/9/10.1182_blood-2004-07-2617/6/zh80090577870006.jpeg?Expires=1735537750&Signature=xcRuRUnSAxmNf~C4Q0aleeNr1IO9pLStk9Z6-ghmV7wICOLDCjoGI8qTiJ8j1Vg2TtSQFmf4MoxV4ntsgeWXMbq1-obfa2MN~nc7CKXrvpsmyh2TUY4bHBsx2KDYwlONGKf~ioeF6REHKZLG8xYb1borSpgOiB1gKgHf-1pWXQHyHukh9dvh55gM8-ruWreuZ5pJKwAl5G~oGaZ13uNWmmkznVv2sXvfNseK-7l216XHyKrvddN~8H14dFjAshn611ZbPumfctVltMQ4rlzYM~yAn9AVsMk0YmCFIglm8PZhdDd4U50QVCyNdoGX~ZiKUUOQLplFK8A1llssMe2p9w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Specificity of the NF-κB–binding site. EMSAs were performed using nuclear protein from HUVECs and biotin-labeled probes (WT [wild-type] oligo, biotin or Mut oligo, biotin) as described in Figure 5. Symbols above each lane (+/-) denote the presence or absence of various reagents. For competition experiments (lanes 4 and 6), a 50-fold excess of unlabeled oligo (WT oligo or Mut oligo) was used. Data are representative of at least 3 independent experiments.
