Specificity of NF-κB binding. EMSAs were performed using nuclear protein from HUVECs as described in Figure 5. Prior to electrophoresis, complexes were incubated with antibodies to either the P50 or P65 subunit of NF-κB. Binding of these antibodies results in a super shift (denoted by +NF-κB Ab) due to the increased mass of the antibody. Data are representative of at least 3 independent experiments.