Role of specific TRAIL receptor triggering in TRAIL-mediated antiadhesive activity and modulation of chemokine release. HUVECs were either left untreated or pretreated for 3 days with the indicated agonistic polyclonal Abs anti-TRAIL receptors before addition of TNF-α. (A) TNF-α-induced HL-60 cell adhesion to HUVECs was set as 100%. Results are expressed as means ± SD of 3 independent experiments, each performed in triplicate. (B) Chemokine release was measured by ELISA. Results are expressed as means ± SD of 3 independent experiments, each performed in triplicate.