The antiadhesive activity of TRAIL is reverted by addition of CXCL10 and CCL8. HUVEC were either left untreated or pretreated for 3 days with TRAIL before addition of TNF-α and adhesion assay. When indicated, recombinant CXCL10 and CCL-8 (A-B), CXCL1 and CCL20 (C), and IL-8 and neutralizing Ab anti-IL-8 (D) were added to the cultures either alone or in combination. TNF-α-induced HL-60 cell adhesion to HUVECs was set as 100%. Results are expressed as means ± SD of 3 to 4 independent experiments, each performed in triplicate.