Figure 5.
Immunohistochemistry analysis to confirm the presence of the expression of the proteins in the MCL samples and confirm the absence of expression differences in other proteins not detected by the protein microarray. Paraffin-embedded tissue biopsies available from the same patients were analyzed using antibodies to human proteins: CRIK-L, Hsp90, and MDM2 (A); Rb2, KU80, Paxillin, and Bcl-x (B). Images were visualized under an Olympus IX70 inverted microscope equipped with objective lenses from 20 ×/0.40 and 60 ×/0.70 (Olympus, Tokyo, Japan). A MagnaFire digital camera (model 599806) and MagnaFire software version 4.1 were used to capture images (Olympus).